Disclaimer: This is purely observational. No judgment or statement here.
Someone who did more work than me and understands how to use the table function... and knew that there was a table function on this board.
Order of the Stick
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Roy Greenhilt: Male, straight, protagonist, leader, love interest of Celia. (459)
Elan: Male, straight, protagonist, love interest of Haley Starshine, Therkla. (432)
Haley Starshine: Female, straight*, protagonist, second-in-command/leader, love interest of Elan. (423)
Belkar Bitterleaf: Male, straight, protagonist. (384)
Vaarsuvius: Androgynous, protagonist, love interest of Inkyrius. (345)
Durkon Thundershield: Male, straight, protagonist, love interest of Hilgya Firehelm, killed. (328)
Team Evil
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Xykon: Male, straight, major antagonist, leader. (94)
Redcloak: Male, straight, major antagonist, second-in-command/leader. (94)
MitD: Male, major character. (66)
Tsukiko: Female, straight (necrophiliac), supporting antagonist, killed. (28)
Jirix: Male, supporting antagonist, leader. (21)
Hobgoblin General: Male, minor antagonist, killed. (5)
Linear Guild
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Nale: Male, straight*, major antagonist, leader, love interest of Sabine. (100)
Sabine: Female, bisexual, major antagonist, second-in-command, love interest of Nale. (72)
thog: Male, major antagonist, killed. (67)
Zz'dtri: Male, supporting antagonist. (43)
Yukyuk: Male, supporting antagonist, killed. (14)
Pompey: Male, straight, supporting antagonist. (11)
Leeky Windstaff: Male, supporting antagonist. (11)
Yokyok: Male, supporting antagonist, killed. (5)
Yikyik: Male, supporting antagonist, killed. (17)
Hilgya Firehelm: Female, straight, supporting antagonist, love interest of Durkon Thundershield. (18)
Azure City
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Shojo: Male, major ally, leader, killed. (35)
Hinjo: Male, major ally, leader. (76)
O-Chul: Male, major ally. (42)
Lien: Female, straight, supporting ally. (38)
Daigo Da-: Male, straight, supporting ally, love interest of Kazumi Kato. (36)
Kazumi Kato: Female, straight, supporting ally, love interest of Daigo Da-. (26)
Miko Miyazaki: Female, straight, major ally/antagonist, killed. (68)
Daimyo Kubota: Male, supporting antagonist, leader, killed. (13)
Therkla: Female, straight, supporting ally/antagonist, love interest of Elan, killed. (20)
General Chang: Male, minor ally, killed. (8)
Sangwaan: Female, minor ally, killed. (6)
Grand Larcenist: Male, minor character, killed. (7)
Team Tarquin
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Tarquin: Male, straight, major antagonist, leader. (66)
Malack: Male, major antagonist, second-in-command. (48)
Empress of Blood: Female, minor antagonist, patsy. (17)
Kilkil: Male , minor antagonist, lackey. (24)
Greysky City
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Bozzok: Male, supporting antagonist, leader. (12)
Hank: Male, supporting antagonist, second-in-command. (9)
Crystal: Female, supporting antagonist, killed. (13)
Jenny: Female, straight, minor antagonist. (6)
Azure City Resistance
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Ho Thanh: Male, supporting ally, leader, killed. (23)
Niu: Female, supporting ally. (18)
Eye-patched leader: Female, minor supporting ally, killed. (7)
Knot-topped leader: Male, minor supporting ally, killed. (6)
Team Peregrine Leader: Androgynous, minor supporting ally, leader, killed. (7)
Team Peregrine Lieutenant: Androgynous, minor supporting ally, second-in-command, killed. (6)
Other
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Celia: Female, straight, supporting ally, love interest of Roy Greenhilt. (71)
Qarr: Male , supporting antagonist. (42)
The IFCC: Males, major antagonists, leaders. (13)
The Oracle: Male, minor character. (10)
Gannji: Male, supporting character, leader. (25)
Enor: Male, supporting character. (23)
Eugene Greenhilt: Male, straight, supporting character, killed. (28)
Julia Greenhilt: Female, supporting character. (16)
Sara Greenhilt: Female, straight, supporting character. (7)
Horace Greenhilt: Male, straight, supporting character. (5)
Ian Starshine: Male, straight, supporting character. (22)
Inkyrius: Androgynous, minor character, love interest of Vaarsuvius. (8)
Mr. Jones: Male, minor character. (17)
Mr. Phil Rodriguez: Male, minor character. (14)
Ancient Black Dragon: Female, straight, supporting antagonist, killed. (11)
Shadowdancer: Male, minor antagonist. (11)
'Kaboom' Redaxe: Male, straight, minor antagonist. (10)
Amun-Zora: Female, straight, minor character. (8)
Chief: Male, minor ally, leader, killed. (10)
Rookie Cop: Male, minor ally, leader. (9)
Greysky Priest: Male, minor ally. (6)
Samantha: Female, straight, minor antagonist, leader, killed. (10)
Samantha's Dad: Male, minor antagonist, second-in-command, killed. (11)
Order of the Scribble
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Soon Kim: Male, straight, minor character, leader. (9)
Lirian: Female, straight, minor character, second-in-command, love interest of Dorukan, killed. (4)
Dorukan: Male, straight, minor character, love interest of Lirian, killed. (4)
Serini: Female, minor character. (3)
Girard Draketooth: Male, minor character. (12)
Kraagor: Male, minor character, killed. (2)
*canonically shown as being heteroflexible or homoflexible, or otherwise interested in broadening their sexual horizons.
Summary
Most important group lead by a woman: Azure City Resistance
Most important group led by a man: Order of the Stick
Most important woman still alive and not a love interest: Lien
Most important man still alive and not a love interest: Belkar Bitterleaf
Most important demonstrably gay person in strip: Sabine
Most important demonstrably straight person in strip: Roy Greenhilt
Gender balance: M: 51, F: 22 (28.57%), A: 4
Not Killed Gender balance: M: 35, F: 13 (26.00%) , A: 2
% of Women Killed: 42.86% (9 killed, 13 not)
% of Men Killed: 31.37% (16 dead, 35 not)
Unattached Gender balance: M: 45, F: 15 (24.19%) , A: 2
% of Unattached Women: 68.18% (15 unattached, 7 attached)
% of Unattached Men: 88.24% (45 unattached, 6 attached)
Leader Gender balance: M: 15, F: 2 (11.11%), A: 1
Appearances Gender Balance: M: 2840, F: 890 (21.73%), A: 366
Non-PC Appearances Gender Balance: M: 1237, F: 467 (27.07%), A: 21
Adjustments
-Sabine is now classified as bisexual, and therefore the most important gay character (since she is the only demonstrably gay character).
-Kilkil is definitely a dude.
-I've included Haley as a leader, considering how she was the on-panel leader of the Azure City Resistance. While the knot-topped and eye-patched were also leaders, they never led on-panel (and frankly, including them as 'characters', let alone leaders, is pretty generous.)
-Xykon is straight as far as we can tell from SOD.
-Azure City Resistance would now be the most important organisation led by a woman.
-According to bonus material, Lien is also straight.
-Added in appearances, as well as appearances gender balances.
-Added in Ian Starshine, Eugene Starshine, Gannji and Enor (characters important enough to have 20+ appearances) and adjusted ratios accordingly.
-Took Shadowknight12's advice and posted % of Wo/Men dead, and % of Wo/Men unattached.
-Added a lot of characters, changed 'dead' to 'killed', readjusted ratios (went more in favour of women with one or two exceptions), added Inkyrius as V's love interest, moved the bandits to 'other', yadda yadda yadda.