Everyone is entitled to their opinion. I've personally always thought of V as male.

Also, make sure that you note this possible typo in Comic #306. The wizards, after spending all day with V helping him write down spells, refer to V as 'he' and 'him.' This occurs considerably after all of the jokes possible have been done to death.

Here's the comic. Check the last panel:
http://www.giantitp.com/comics/oots0306.html